| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Acidithiobacillus ferrooxidans | TTCGCTTGGTACAGCAGGC | TCCGCACGCACTTCAACT | 60.67 | 59.89 | 214 | 138 |
100.00%
|
100.00%
|
| Primer 12 | Acidithiobacillus ferrooxidans | GCGCTACTTTGACGAGCAG | TCCGCACGCACTTCAACT | 59.30 | 59.89 | 197 | 138 |
100.00%
|
100.00%
|
| Primer 13 | Acidithiobacillus ferrooxidans | TTGGGTTGAGTGGCTGCA | TTTTGTCACCGCAGCACC | 59.40 | 58.88 | 242 | 138 |
100.00%
|
100.00%
|
| Primer 14 | Acidithiobacillus ferrooxidans | TGCTGCTGCGTCTGGAAA | GCAGCGCTTTGAGTTGCA | 59.89 | 59.67 | 252 | 137 |
100.00%
|
100.00%
|
| Primer 15 | Acidithiobacillus ferrooxidans | TGCGCACATGGTCGATGT | GGTCCTTTTGGTGGCCTGT | 60.05 | 60.15 | 172 | 137 |
100.00%
|
100.00%
|
| Primer 16 | Acidithiobacillus ferrooxidans | TGCGCACATGGTCGATGT | AGGTCCTTTTGGTGGCCTG | 60.05 | 59.85 | 173 | 137 |
100.00%
|
100.00%
|
| Primer 17 | Acidithiobacillus ferrooxidans | GTTCACGGTATCGGTCCCA | AAAGCGTTCGTCTGCGGA | 59.11 | 59.97 | 194 | 135 |
100.00%
|
100.00%
|
| Primer 18 | Acidithiobacillus ferrooxidans | TTGACGATGCGCTGACCA | TCACGCTGGCGAATCAGT | 59.66 | 59.34 | 223 | 135 |
100.00%
|
100.00%
|
| Primer 19 | Acidithiobacillus ferrooxidans | TTGACGATGCGCTGACCA | TCCTGGTCAAACGCGTTGT | 59.66 | 60.15 | 205 | 135 |
100.00%
|
100.00%
|
| Primer 20 | Acidithiobacillus ferrooxidans | TTGACGATGCGCTGACCA | GGTCAAACGCGTTGTCGAG | 59.66 | 59.80 | 201 | 135 |
100.00%
|
100.00%
|